The extension of Rayleigh’s theory to describe the scattering of light by larger macromolecules in solution is called the Rayleigh-Gans-Debye (RGD) theory of light scattering. His insights were based on the fundamental equations describing light and its interaction with matter, laid out by James Clerk Maxwell in 1865 in one of the most important achievements of theoretical physics. In the 19th century, Lord Rayleigh (John William Strutt) offered the first explanation for the sky's brilliant blue color (on a clear day!). Dynamic & Electrophoretic Light Scattering.For more infromation see Usage Policies.Please Note - Since repairs/service from normal wear might not be necessary every year, depending on use, these costs might come quite some time after use.Cost for repairs/parts/services resulting from normal wear will be split based on the time used between all user groups.Users are expected to pay for repairs or parts that are necessary due to damage caused by their carelessness or neglect.Most columns used frequently for 'normal' gel filtration application will be unsuitable for SEC-MALS.SEC columns for Light Scattering must be in top condition and should not 'bleed' any column material.Users are expected to replace any borrowed columns that were damaged by them Upon request it might be possible to borrow columns from other user groups.Users are expected to provide their own SEC columns.Users are expected to provide all consumables specific to their experiment.For detailed information please contact Dr. Regular user groups are expected to opt into the pool and pay an monthly share that goes towards the general up keep of the Shared Instruments Pool.

In CG-MALS a composition-gradient preparative system is combined with MALS/RI/UV detectors to measure interactions between biological macromolecules, by directly observing changes in molar mass due to complex formation or dissociation in solution and without the need for labeling or immobilization. In contrast to conventional SEC chromatography this method can accurately measure MW, even for sticky or elongated proteins that elute to late or too early for their actual mass to be correctly determined by size exclusion chromatography SEC alone (due to their unusual interaction with the columns).Īn integrated dynamic light scattering detector allows measurement of the hydrodynamic radius (R h) of particles, with a lower limit of 0.5 nm. SEC-MALS can be used to determine the absolute molecular mass of proteins and complexes in complex samples, independent of their elution volume from the SEC column. SEC-MALS uses size exclusion chromatography (SEC) coupled to an in-line multi- angle light scattering detector and an in-line refractive index or UV/Vis detector. MALS in conjunction with a concentration detector can directly measure the absolute molar masses of macromolecules and complexes, independent of structure and shape from 200 daltons to hundreds of millions of daltons.

Static vs dynamic light scattering